Applied Biomics

read moreApplied Biomics is dedicated to providing top-quality, cost-effective and full-line Proteomics Services, from sample preparation, protein array, mass spectrometry to high quality data for publications. All our proteomics services are performed in house. Quantitative proteomics reveal lineage-specific protein profiles in iPSC-derived Marfan syndrome smooth muscle cells. Hydrogen Sulfide Therapy Suppresses Cofilin-2 and Attenuates Ischemic Heart Failure in a Mouse Model of Myocardial Infarction. Glucose-6-phosphate dehydrogenase increases Ca2+ currents by interacting with Cav1.2 and reducing intrinsic inactivation of the L-type calcium channel.

read moreApplied Biomics, Inc., is a Proteomics Service company established in 2005. We are centrally located in San Francisco bay area, and occupy over 5,000 sq ft lab and office spaces. We are dedicated to providing top-quality, cost-effective and full-line proteomics services to meet our customers' research and discovery needs. We cover both major platforms of proteomics: gel-based 2D DIGE (2-dimensional differential in-gel electrophoresis) and liquid-based iTRAQ (isobaric tags for relative and absolute quantitation).

read moreOur Mass Spectrometry services offer almost 100% Success Rate in protein identification, by employing the lastest technologies and optimized protocols. A. MALDI TOF/TOF Procedures (Please follow these Guidelines in preparing and shipping samples): In each run, four sensitivity standards in the amount of 1 femtomole are used to monitor the assay performance. 1. Gel treatment: 2D gel spots are washed multiple times to remove staining dye and other inhibitory chemicals. Gel spots are then dried to absorb maximum volume of digestion buffer.

read moreNano LC-MS/MS Proteomics Analysis can identify all proteins in a mixture ranging from 1D gel bands, IP samples to whole cell lysate or tissues. 1 Protein fractionations: Fractionate proteins by either pI or Molecular weight at your choice. This is very useful for tissues, cell lysates, or culture media. 2 Depletion of serum abundant proteins: Please note that LC-MS/MS detection of serum low abundant proteins is limited even after depletion of serum abundant proteins. We highly recommend Serum 2D DIGE for all serum and plasma samples.

read moreOur 2D DIGE Protein Array / Mass Spectrometry proteomics services offer a powerful platform in studying the high complexity proteome of all biological systems. While 2D gel continues to be one of the most versatile and widely used techniques, a modified version, 2D-DIGE, which labels different protein samples with up to three fluorescent tags, offers greater sensitivity and reproducibility over conventional 2D-PAGE gels. 1. Sample preparation: Proteins are extracted from cells or tissues of interest.

read moreApplied Biomics offers the most comprehensive HCP Antibody Coverage analysis by using high resolution large format 2D fluorescent Western Blot. Our report provides HCP, Western blot and HCP/Western overlay images, allowing the direct visualization of antibody coverage that no other platform could achieve. In addition, the HCP antibody coverage is calculated with high consistency and accuracy (see details below). 1 High consistency: Fluorescent 2D Western detects both protein and WB images from the same gel and same membrane, allowing the perfect alignments between protein and WB spots, thus eliminating any gel-to-gel or gel-to-membrane variations as seen in the traditional 2D WBs.